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Mould growth on black table olives and prevention by sorbic acid, methyl-eugenol and spice essential oil
1Food Science Department, Faculty of Agriculture, Atatürk University, Erzurum, Turkey.
Abstract:
Thirty-two mould strains were isolated from Turkish-style black table olives. Four of these isolates were identified as Aspergillus ochraceus, two as A. niger, 16 as Penicillium clavigerum and 10 as P. expansum. Mould growth was evaluated on olives treated with 500 ppm sorbic acid, 1000 ppm methyl-eugenol and 1500 ppm çörtük spice (Echinophora sibthorpiana Guss.) essential oil. Olives inoculated with A. parasiticus NRRL 2999 and P. clavigerum were also examined. All samples were stored at 28 degrees C, 15 degrees C and 5 degrees C for 60 days. The lower the temperature, the more time it took the mould to initiate mycelium and spore development. Most inhibition of growth was obtained using sorbic acid in all cases and especially at low temperatures, followed by methyl-eugenol and essential oil. Decreased inhibition was observed in noninoculated olives. Inhibitory effect of used agents was higher in olives inoculated with P. clavigerum than in olives inoculated with A. parasiticus.
Insights
Sorbic acid, methyl-eugenol, and çörtük spice essential oil effectively inhibited mould growth on black table olives. Lower temperatures significantly slowed mould development, with sorbic acid showing the most potent antimicrobial effect.
Area of Science:
- Food Microbiology
- Mycology
- Food Preservation
Background:
- Mould contamination poses a significant challenge to the shelf-life and safety of Turkish-style black table olives.
- Effective preservation strategies are crucial to prevent spoilage and mycotoxin production.
Purpose of the Study:
- To evaluate the efficacy of sorbic acid, methyl-eugenol, and çörtük spice essential oil in inhibiting mould growth on black table olives.
- To assess the impact of storage temperature on mould development and the effectiveness of the tested agents.
Main Methods:
- Isolation and identification of native mould strains from Turkish olives, including Aspergillus and Penicillium species.
- Treatment of olives with sorbic acid, methyl-eugenol, and çörtük spice essential oil, followed by inoculation with specific mould strains (A. parasiticus, P. clavigerum).
- Storage of treated and inoculated olives at different temperatures (28°C, 15°C, 5°C) for 60 days and evaluation of mould growth.
Main Results:
- Sorbic acid demonstrated the highest inhibitory effect on mould growth across all conditions, particularly at lower temperatures.
- Methyl-eugenol and çörtük spice essential oil also exhibited significant mould inhibition, though less potent than sorbic acid.
- Lower storage temperatures (5°C) markedly delayed mycelium and spore development compared to higher temperatures (28°C).
- The inhibitory effect of the agents was more pronounced on olives inoculated with P. clavigerum compared to A. parasiticus.
Conclusions:
- Sorbic acid, methyl-eugenol, and çörtük spice essential oil are effective agents for controlling mould growth in Turkish-style black table olives.
- Low-temperature storage is a critical factor in extending the shelf-life by slowing down mould proliferation.
- The choice of preservative and storage conditions can be optimized based on the target spoilage microorganisms.
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