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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
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Related Experiment Video

Updated: May 9, 2026

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
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Antigen specific immunotherapy generates CD27(+) CD35(+) tolerogenic dendritic cells.

Zhiqiang Wu1

  • 1Department of Gastroenterology, First Affiliated Hospital, The PLA General Hospital, Beijing 100037, China. zhiqiangwu32@163.com

Cellular Immunology
|July 27, 2013
PubMed
Summary

Antigen-specific immunotherapy (ASIT) for food allergies (FA) promotes the generation of tolerogenic dendritic cells (DCs). This involves CD27(+) B cells producing TGF-b, which induces regulatory T cells, offering a new therapeutic understanding.

Keywords:
Antigen specific immunotherapyB cellCD27CD35Food allergy

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Area of Science:

  • Immunology
  • Allergy Research
  • Cellular Biology

Background:

  • The precise mechanisms of antigen-specific immunotherapy (ASIT) require further elucidation.
  • Understanding ASIT's role in modulating immune responses, particularly in food allergy (FA), is crucial.

Purpose of the Study:

  • To investigate the role of ASIT in generating tolerogenic dendritic cells (DCs) in patients with FA.
  • To explore the cellular and molecular pathways involved in ASIT-induced immune tolerance.

Main Methods:

  • Flow cytometry analysis of blood samples from 20 FA patients and 10 healthy controls before and after ASIT.
  • Coculture experiments involving CD27(+) CD35(+) B cells and immature DCs.

Main Results:

  • FA patients exhibited lower frequencies of CD27(+) B cells and TGF-b(+) DCs compared to healthy subjects.
  • CD27(+) B cells, often expressing CD35, produced TGF-b.
  • Coculture demonstrated that CD27(+) CD35(+) B cells induced TGF-b-expressing tolerogenic DCs, which in turn generated regulatory T cells.

Conclusions:

  • ASIT treatment effectively induces TGF-b-expressing tolerogenic DCs in individuals with FA.
  • This study highlights a novel mechanism by which ASIT may restore immune tolerance in food allergies.