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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: May 9, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Detecting and comparing non-coding RNAs in the high-throughput era.

Giovanni Bussotti¹, Cedric Notredame, Anton J Enright

¹European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK. giovanni.bussotti@ebi.ac.uk

International Journal of Molecular Sciences

|July 27, 2013

Summary

This summary is machine-generated.

Discovering non-coding RNA (ncRNA) requires advanced sequence alignment methods. This review explores new techniques for comparing ncRNA sequences and analyzing high-throughput data, crucial for understanding cellular functions.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Growing interest in non-coding RNA (ncRNA) due to discovery of new genes and their cellular roles.
Accurate detection and comparison of RNA sequences are vital for identifying homologous genes and biological patterns.
Comparing ncRNAs is challenging due to limited sequence conservation and evolution constrained by structure.

Purpose of the Study:

To review comparative genomics of non-coding RNAs.
To focus on new sequencing technologies and their impact on ncRNA analysis.
To address the need for novel methods in RNA alignment and high-throughput data analysis.

Main Methods:

Review of current literature on ncRNA comparative genomics.
Discussion of challenges in aligning RNA sequences, particularly non-coding ones.
Exploration of new methods for RNA sequence alignment and high-throughput data analysis.

Main Results:

Highlighting the limitations of traditional sequence alignment for ncRNAs.
Emphasizing the importance of structural conservation in ncRNA evolution.
Identifying the need for advanced computational tools to analyze ncRNA data.

Conclusions:

New methods for RNA alignment are urgently needed to fully exploit experimental data.
Advancements in sequencing technologies necessitate improved bioinformatics approaches for ncRNA research.
Effective comparative genomics of ncRNAs will enhance our understanding of their roles in cellular functions.