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Stability and antimicrobial property of soy protein/chitosan mixed emulsion at acidic condition
Yang Yuan1, Zhili Wan, Shouwei Yin
1Research and Development Center of Food Proteins, College of Light Industry and Food, South China University of Technology, Guangzhou 510640, PR China.
Abstract:
To fabricate a soy protein emulsion with good storage stability against microorganisms, we investigated the stability and antimicrobial activity of a β-conglycinin (7S) and chitosan (CS) mixed emulsion at acidic pHs. Results of droplet size and microstructure showed that 7S/CS mixed emulsions were stable at acidic pHs while the 7S emulsion was not. As for the storage test, results showed that the 7S/CS mixed emulsion displayed an significantly (p < 0.05) improved storage stability than the control both under un-inoculated and inoculated conditions at 20 °C because of the presence of chitosan. A well diffusion assay showed that the 7S/CS mixed emulsion provided better inhibition against Escherichia coli and the minimum mixing ratio of the chitosan content, of 0.25 mg mL(-1), adequately inhibited the bacteria used in this research. Moreover, the aerobic plate count analysis indicated that the 7S/CS mixed emulsion, compared to the control, effectively inhibited the growth of microorganisms. This inhibition was believed to be independent of the CS/7S mixing ratio. The results in this paper demonstrate that the growth of microorganisms dramatically accelerated the destabilization of the stable 7S emulsion and the fabrication of 7S/CS mixed emulsion could increase the acidic stability and antimicrobial activity in order to extend shelf-life.
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