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Clonidine and glucagon stimulation for testing growth hormone secretion in children and adolescents: can we make it
A Christoforidis1, P Triantafyllou, A Slavakis
11st Paediatric Department, Aristotle University, Thessaloniki, Greece. christoforidis @doctors.org.uk.
Background:
Our aim was to retrospectively evaluate the possibility to reduce the number of GH analyses during clonidine and glucagon tests without compromising accuracy.
Subjects And Methods:
Two hundred and forty-five tests were performed in a total of 188 children and adolescents with a mean age of 9.93 ± 2.88 yr in a single center during the last 5 yr.
Results:
Ninety-one out of 158 (57.59%) clonidine tests and 47/87 (54.02%) glucagon tests had at least one sample >10 μg/l (negative). For clonidine tests, not measuring GH at 30 min would have resulted in only one negative test missed (0.63% false positive result), whereas not measuring GH both at 0 and 30 min would have increased the false positive percentage to 2.53%. Ending clonidine tests at 90 min would have resulted in 7 negative tests missed (4.43% false positive results). For glucagon tests, more than half of the tests peaked at 120 min (56.32%). Skipping sampling at 0, 60 and 180 min provided a false positive rate of 5.75%.
Conclusions:
For clonidine tests we can omit blood sampling at time points 0 and 30 min without significantly compromising accuracy.
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