Related Experiment Video
Updated: May 9, 2026
12:04
Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Genome engineering with TAL-effector nucleases and alternative modular nuclease technologies
Andrew M Scharenberg1, Philippe Duchateau, Julianne Smith
1University of Washington School of Medicine, Seattle Children's Research Institute, Seattle, WA, USA. scharenberg@cellectis.com.
Current Gene Therapy
|July 30, 2013
Summary
TAL effector and modular DNA binding domain (mDBD) technologies are key for site-specific DNA binding in biological research. These advancements are driving new genome engineering tools and future therapeutic applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- TAL effector DNA binding domains are a leading platform for creating site-specific DNA binding polypeptides.
- Modular DNA binding domains (mDBDs) offer alternative strategies for genome engineering.
Purpose of the Study:
- To provide an overview of TAL effector and mDBD technologies for genome engineering.
- To discuss the selection criteria for these technologies in various applications.
- To highlight the evolution towards next-generation genome engineering tools.
Main Methods:
- Review of TAL effector and mDBD technologies.
- Analysis of established and emerging nuclease architectures.
- Discussion of application considerations from cell lines to therapeutics.
Main Results:
- TAL effectors and mDBDs are crucial for building site-specific nucleases.
- These technologies are applicable in diverse genome engineering projects.
- Rapid development has shifted focus to post-cleavage control mechanisms.
Conclusions:
- TAL effector/mDBD technologies are foundational for current genome engineering.
- The field is advancing towards controlling events after DNA breaks.
- Next-generation technologies are emerging for sophisticated genome manipulation.
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