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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Tumor-suppressive microRNA-1291 directly regulates glucose transporter 1 in renal cell carcinoma

Takeshi Yamasaki1, Naohiko Seki, Hirofumi Yoshino

  • 1Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.

Cancer Science
|July 30, 2013
PubMed

Insights

MicroRNA-1291 (miR-1291) acts as a tumor suppressor in renal cell carcinoma (RCC). Its downregulation promotes RCC cell proliferation and metastasis by targeting SLC2A1/GLUT1, offering new therapeutic insights.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-1291 (miR-1291) is downregulated in renal cell carcinoma (RCC).
  • miR-1291 is a putative tumor-suppressive miRNA in RCC.
  • The functional role and molecular targets of miR-1291 in RCC remain to be fully elucidated.

Purpose of the Study:

  • To investigate the functional significance of miR-1291 in RCC.
  • To identify novel miR-1291-mediated cancer pathways and target genes in RCC.

Main Methods:

  • Gene expression analysis of RCC clinical specimens and miR-1291-transfected cells.
  • In silico database analysis.
  • Luciferase reporter assays to confirm direct target regulation.

Main Results:

  • miR-1291 restoration inhibited RCC cell proliferation, migration, and invasion.
  • 79 signaling pathways were significantly regulated by miR-1291.
  • Solute carrier family 2 member 1 (SLC2A1), encoding glucose transporter protein type 1 (GLUT1), was identified as a direct target of miR-1291.
  • SLC2A1/GLUT1 expression was inversely correlated with miR-1291 levels in RCC tissues.

Conclusions:

  • miR-1291 functions as a tumor suppressor in RCC.
  • The miR-1291/SLC2A1/GLUT1 axis plays a critical role in RCC oncogenesis and metastasis.
  • Targeting this pathway may offer novel therapeutic strategies for RCC.

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