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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Related Experiment Video

Updated: May 9, 2026

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Plasma cells for hire: prior experience required.

James Hagman¹, Kara Lukin

¹Integrated Department of Immunology, K516B, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA. hagmanj@njhealth.org

|July 30, 2013

Summary

Reduced Bach2 in memory B cells drives robust IgG1 antibody production during secondary immune responses. This finding is crucial for understanding antibody generation and immune memory.

Area of Science:

Immunology
Molecular Biology

Background:

Memory B cells are essential for rapid and potent secondary antibody responses.
These cells differentiate into antibody-secreting plasma cells upon re-exposure to an antigen.

Purpose of the Study:

To investigate the role of the transcription factor Bach2 in regulating IgG1 antibody production by memory B cells.
To understand the molecular mechanisms controlling plasma cell differentiation in secondary immune responses.

Main Methods:

Analysis of Bach2 expression levels in antigen-experienced B cells.
Genetic manipulation of Bach2 in mouse models.
Flow cytometry to quantify plasma cell populations and antibody titers.

Main Results:

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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

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Lowered Bach2 levels were observed in antigen-experienced memory B cells.

Reduced Bach2 expression correlated with increased differentiation into IgG1-producing plasma cells.

Bach2 deficiency led to enhanced IgG1 antibody production in secondary responses.

Conclusions:

Bach2 acts as a negative regulator of IgG1 plasma cell differentiation.
Modulating Bach2 levels in memory B cells can control antibody production during secondary immune responses.