Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
What are Second Messengers?01:12

What are Second Messengers?

Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
Signal Transduction: Overview01:26

Signal Transduction: Overview

Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medical students' perspectives on body donation to science within the Italian context.

Anatomical sciences education·2026
Same author

Corrigendum to "Synthesis and biological evaluation of mixed aryl-alkyl succinates as modulators of autophagy and apoptosis in gastric carcinoma" [Bioorg. Chem. 179 (2026) 109997].

Bioorganic chemistry·2026
Same author

Advances in understanding the roles of TP53 mutations in pancreatic and hepatobiliary cancers.

Advances in biological regulation·2026
Same author

Synthesis and biological evaluation of mixed aryl-alkyl succinates as modulators of autophagy and apoptosis in gastric carcinoma.

Bioorganic chemistry·2026
Same author

Nuclear phosphoinositides: An exploration into their regulation, roles and physico-chemical environment.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Foreword.

Advances in biological regulation·2026

Related Experiment Video

Updated: May 9, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Nuclear inositide specific phospholipase C signalling - interactions and activity.

Irene Faenza1, Roberta Fiume, Manuela Piazzi

  • 1Cell Signaling Laboratory, Department of Biomedical Science (DIBINEM), University of Bologna, Italy.

The FEBS Journal
|July 30, 2013
PubMed
Summary

Nuclear inositol lipid metabolism, including phosphatidylinositol 4,5-bisphosphate signaling, operates independently within the nucleus. Nuclear phospholipase C (PI-PLC) enzymes are key regulators of this autonomous system, influencing cell differentiation and proliferation.

Keywords:
inositide specific phospholipase Cinteractomelocalizationnucleussignalling

More Related Videos

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

Related Experiment Videos

Last Updated: May 9, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
10:31

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Accumulating evidence over 20 years indicates autonomous nuclear inositol lipid metabolism.
  • Lipid signaling molecules are integral to nuclear signaling pathways.
  • Nuclear polyphosphoinositide (PI) signaling involves phosphatidylinositol 4,5-bisphosphate synthesis and metabolism.

Purpose of the Study:

  • To review the signal transduction-related metabolism of nuclear inositide-specific phospholipase C (PI-PLC).
  • To present evidence for nuclear PI-PLC involvement in cell differentiation and proliferation.
  • To highlight the nucleus as a distinct compartment for autonomous inositol lipid metabolism.

Main Methods:

  • Literature review focusing on nuclear PI-PLC expression and activity.
  • Analysis of studies investigating nuclear inositol lipid metabolism.
  • Examination of PI-PLC isoforms (β1, γ1, δ1, ζ) within the nucleus.

Main Results:

  • Nuclear PI pool regulation is independent of the plasma membrane.
  • Several PI-PLC isoforms are present in the nucleus, with PI-PLC-β1 being the most characterized.
  • Nuclear PI-PLC acts as an effector and interactor in nuclear inositide signaling.

Conclusions:

  • The inositide cycle operates with a biological role within the nucleus.
  • Nuclear inositol lipid metabolism represents an autonomous, lipid-dependent signaling system.
  • This nuclear system is independently regulated and involved in cell cycle progression and differentiation.