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Updated: May 9, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates
Lorena Cristina Corrêa Fehlberg1, Lucas Henrique Sales Andrade, Diego Magno Assis
1Departamento de Medicina, Laboratório Alerta, Universidade Federal de São Paulo, São Paulo, Brazil.
Abstract:
We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%).
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