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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR01:32

PCR

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Updated: May 9, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in

Chelsie N Geyer1, Nancy D Hanson

  • 1Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology (C.R.A.B), 2500 California Plaza, Omaha, NE 68178, USA.

Diagnostic Microbiology and Infectious Disease
|July 30, 2013
PubMed
Summary
This summary is machine-generated.

Rapid PCR amplification significantly reduced detection times for β-lactamase genes, enabling faster identification of antimicrobial resistance mechanisms in Gram-negative bacteria.

Keywords:
Beta-lactamase genesDiagnostic PCRRapid amplification

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Clinical Diagnostics

Background:

  • Antimicrobial resistance is a growing global health threat.
  • Rapid and accurate detection of resistance mechanisms is crucial for effective treatment.
  • β-lactamase genes are a primary mechanism of resistance to β-lactam antibiotics.

Purpose of the Study:

  • To evaluate the efficiency of rapid PCR amplification for detecting β-lactamase genes.
  • To assess the potential of this method in clinical microbiology.

Main Methods:

  • Utilized a previously designed end-point multiplex PCR assay and singleplex assays.
  • Employed rapid PCR amplification methodology for gene detection.
  • Measured amplification and overall detection times.

Main Results:

  • Achieved rapid PCR amplification times of 16–18 minutes.
  • Completed overall detection within 1.5 hours.
  • Demonstrated a significant decrease in time compared to conventional methods.

Conclusions:

  • Rapid PCR amplification is a viable method for the swift detection of β-lactamase genes.
  • This methodology can expedite the identification of antimicrobial resistance mechanisms in Gram-negative organisms.
  • Faster diagnostics can lead to improved patient management and infection control strategies.