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Updated: May 9, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Pablo Perez-Pinera1, D Dewran Kocak, Christopher M Vockley
1Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.
Abstract:
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
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