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Magnesium oxide grafted carbon nanotubes based impedimetric genosensor for biomedical application
Manoj Kumar Patel1, Md Azahar Ali, Saurabh Srivastava
1Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India; Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
A novel genosensor was developed using nanostructured magnesium oxide grafted carboxyl functionalized multi-walled carbon nanotubes for detecting Vibrio cholerae. This DNA-based sensor offers high sensitivity and stability for biomedical applications.
Area of Science:
- Nanotechnology
- Biosensors
- Molecular Diagnostics
Background:
- Vibrio cholerae poses a significant global health threat, necessitating rapid and sensitive detection methods.
- Existing detection techniques often lack the required sensitivity, speed, or portability for widespread use.
- Carbon nanotube-based nanomaterials offer unique electrochemical properties suitable for biosensor development.
Purpose of the Study:
- To develop a novel impedimetric genosensor for the detection of Vibrio cholerae.
- To functionalize a nanostructured magnesium oxide grafted carboxyl functionalized multi-walled carbon nanotube (nMgO-cMWCNTs) platform with a specific DNA probe.
- To characterize the fabricated sensor and evaluate its performance for Vibrio cholerae detection.
Main Methods:
- Electrophoretic deposition of nMgO-cMWCNTs onto an indium tin oxide (ITO) coated glass electrode.
- Covalent immobilization of an aminated 23-base single-stranded DNA (NH2-ssDNA) probe sequence (O1 gene) of V. cholerae using EDC-NHS chemistry.
- Characterization using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and electrochemical impedance spectroscopy (EIS).
Main Results:
- XPS studies confirmed the presence of O-C=O groups on the nMgO-cMWCNTs surface for effective DNA binding.
- Electrochemical impedance spectroscopy (EIS) revealed a sensitivity of 3.87 Ω ng(-1) cm(-2) and a detection limit of approximately 21.70 ng µL(-1) within the linear range of 100-500 ng µL(-1).
- The developed genosensor demonstrated excellent stability, maintaining its performance for approximately 120 days.
Conclusions:
- The DNA-functionalized nMgO-cMWCNTs nanomatrix serves as a promising impedimetric platform for genosensor fabrication.
- The developed genosensor offers a novel, compact, and sensitive approach for Vibrio cholerae detection.
- This technology holds potential for future development of advanced genosensor devices for various biomedical applications.
