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Scattering detection using a photonic-microfluidic integrated device with on-chip collection capabilities.

Benjamin R Watts1, Zhiyi Zhang, Chang Qing Xu

  • 1Department of Engineering Physics, McMaster University, Hamilton, Canada.

Electrophoresis
|July 30, 2013
PubMed
Summary

SU-8-based photonic-microfluidic devices with integrated beam shaping offer precise scattering detection. These devices achieve excellent coefficient of variation (CV) values for bead counting, demonstrating reliable performance for flow cytometry applications.

Keywords:
Integrated opticsLab-on-a-chipMicrofluidic devicesOptofluidicsScatter detection

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Area of Science:

  • Photonics
  • Microfluidics
  • Optical Detection

Background:

  • Photonic-microfluidic integrated devices are crucial for advanced sensing applications.
  • On-chip beam shaping and collection are key to improving detection sensitivity and reliability.
  • Scattering detection and counting applications require precise control over optical parameters.

Purpose of the Study:

  • To demonstrate SU-8-based photonic-microfluidic devices with on-chip beam shaping and collection.
  • To evaluate the performance of these devices in scattering detection and counting applications.
  • To assess the impact of on-chip beam geometry and bead size on detection CV values.

Main Methods:

  • Fabrication of SU-8-based photonic-microfluidic devices with integrated excitation optics.
  • Utilizing tailored beam geometries for scattering detection of micro-beads.
  • Employing both free-space and on-chip optical waveguide collection schemes.
  • Analyzing device performance using coefficient of variation (CV) and coincident rates.

Main Results:

  • Excellent CV values (11.0-16.4%) achieved for 1-5 μm blank beads with free-space detection.
  • Demonstrated ability to resolve mixed populations of 2.0 and 5.0 μm beads with low CVs (15.9% and 18.5%).
  • On-chip collection via optical waveguides yielded a high coincident rate of 94.2% compared to free-space collection.
  • Achieved a CV of 19.2% for 5 μm beads using on-chip excitation and collection with a 6.0-μm beam waist.

Conclusions:

  • SU-8-based photonic-microfluidic devices enable effective scattering detection and counting with on-chip beam shaping.
  • Device performance is significantly influenced by the interplay between on-chip beam geometry and bead size.
  • On-chip optical waveguide collection offers superior reliability over free-space methods for these integrated devices.