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Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
Published on: January 24, 2017
Automated formation of multicomponent-encapuslating vesosomes using continuous flow microcentrifugation
Huisoo Jang1, Peichi C Hu, Sungho Jung
1Department of Biological Engineering, Inha University, Incheon, Republic of Korea; Biohybrid Systems Research Center, Inha University, Incheon, Republic of Korea.
Biotechnology Journal
|July 30, 2013
Summary
Automated microfluidics and centrifugation create uniform vesosomes, offering a scalable method for cellular compartmentalization models. This platform simplifies the fabrication of complex carriers and artificial cells.
Area of Science:
- Biomaterials Science
- Cellular Engineering
- Microfluidics
Background:
- Vesosomes, hierarchical assemblies of vesicles, show promise as models for cellular compartmentalization.
- Existing vesosome fabrication methods are manual, time-consuming, and lack control over product size and uniformity.
Purpose of the Study:
- To develop an automated platform for vesosome fabrication using microfluidics and continuous flow centrifugation.
- To achieve precise control over vesosome size and uniformity for applications in model cell development.
Main Methods:
- A microfluidic device formed water-in-oil droplets containing nanoscale vesicles.
- These droplets were processed in a continuous flow microcentrifuge to create bilayer-encapsulated vesosomes.
- Fluorescence microscopy and laser diffraction analyzed vesicle encapsulation and uniformity.
Main Results:
- The automated platform successfully generated uniform vesosomes with a coefficient of variation of 0.029.
- The microcentrifuge achieved a high yield, converting over 60% of droplets into vesosomes.
- Encapsulation of nanoscale liposomes within the outer vesosome membrane was confirmed.
Conclusions:
- A fully automatable system for generating vesosomes with controlled contents was developed.
- The microfluidic and centrifugation method offers a simple, scalable platform for fabricating multicomponent carriers and model cells.
- This approach facilitates the creation of artificial cells and complex vesicular systems.
