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Differential protein expression in perfusates from metastasized rat livers
Yang Zhang1,2, Menglin Li1, Lilong Wei1
1National Key Laboratory of Medical Molecular Biology, Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing 100005, China.
Proteome Science
|July 31, 2013
Summary
Liver perfusates offer a promising source for discovering disease biomarkers. This study identified differentially expressed proteins in metastasized liver perfusates, highlighting potential diagnostic markers for liver diseases.
Area of Science:
- Proteomics
- Biomarker Discovery
- Liver Disease Research
Background:
- Liver perfusates contain proteins that enter the bloodstream, offering theoretical advantages for biomarker discovery.
- Perfusion techniques preserve the disease state in its natural context, enhancing biomarker relevance.
- Investigating liver perfusate proteome is crucial for identifying novel biomarkers in liver diseases.
Purpose of the Study:
- To explore the utility of liver perfusate proteome in discovering biomarkers for liver diseases.
- To identify differentially expressed proteins in liver perfusates from tumor-bearing rat livers.
Main Methods:
- Proteomic analysis of isolated rat liver perfusates from livers metastasized by Walker-256 tumor cells.
- Identification and quantification of differentially expressed proteins.
- Bioinformatic analysis of protein functions and pathways.
- Validation of specific protein abundance using Western blotting.
Main Results:
- Identified 86 differentially expressed proteins in metastasized liver perfusates.
- Secretory proteins were predominantly decreased, indicating impaired acute phase response.
- Intracellular/membrane proteins, including cancer-related proteins like Ywhab, showed increased abundance.
- Ywhab (14-3-3 protein family) showed a 3.5-fold increase in perfusates and was validated (3.7-fold increase) in plasma.
Conclusions:
- Liver perfusate proteome serves as a valuable alternative resource for initial biomarker identification.
- Identified proteins, particularly Ywhab, show potential as biomarkers for liver metastasis.
- Further validation of perfusate biomarkers in patient serum is essential.
