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Updated: May 9, 2026

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
Published on: June 27, 2015
[11R-VIVIT inhibits the expression of urokinase-type plasminogen activator receptor in podocytes]
1Department of Nephrology, Guangdong Academy of Medical Sciences, Guangzhou, China. 15920309398@139.com
Objective:
To observe the effect of 11R-VIVIT on lipopolysaccharide (LPS)-induced expression of urokinase-type plasminogen activator receptor (uPAR) in podocytes.
Methods:
A LPS-induced proteinuria mouse model and in vitro cultured podocytes treated with LPS were both divided into control group, LPS group and LPS+11 R-VIVIT group. The mRNA and protein expressions of uPAR in mouse kidney tissues and the podocytes were measured by real-time qPCR, laser scanning confocal microscopy and Western blotting.
Results:
Compared with LPS group, LPS+11 R-VIVIT group showed a significantly lowered urine albumin/creatinine ratio (P<0.001) and markedly reduced mRNA and protein expressions of uPAR (PuPAR mRNA<0.001; PuPAR=0.001).
Conclusion:
11R-VIVIT can ameliorate proteinuria probably by decreasing the expression of uPAR in podocytes.
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