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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Related Experiment Video

Updated: May 9, 2026

Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

[Flow cytometry: is it a novel tool in microbiological diagnostics?].

Eva Pállinger1

  • 1Semmelweis Egyetem, Általános Orvostudományi Kar Genetikai, Sejt- és Immunbiológiai Intézet Budapest. eva.pallinger@gmail.com

Orvosi Hetilap
|July 31, 2013
PubMed
Summary

Rapid microbiological testing methods are needed. Fluorescence-activated cell sorting (FACS) offers a faster, more sensitive approach, identifying individual microbial cells and host immune status, unlike nucleic acid methods.

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Last Updated: May 9, 2026

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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
13:28

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

Area of Science:

  • Microbiology
  • Immunology
  • Biotechnology

Context:

  • Direct pathogen detection is time-consuming and labor-intensive.
  • Conventional microbiological testing methods face limitations in speed and sensitivity.
  • There's a growing need for advanced diagnostic tools in clinical microbiology.

Purpose:

  • To explore advanced microbiological testing methods beyond conventional techniques.
  • To highlight the advantages of Fluorescence-Activated Cell Sorting (FACS) in pathogen detection and host immune monitoring.
  • To address the limitations of nucleic acid-based methods in clinical diagnostics.

Summary:

  • Nucleic acid-based detection methods accelerate diagnosis and enhance specificity but do not provide host immune status information.
  • Detection of pathogen-specific nucleic acids does not confirm the presence of viable microbes.
  • Fluorescence-activated cell sorting (FACS) can identify individual microbial cells, monitor host immune status, and track antimicrobial therapy.

Impact:

  • FACS offers a significant advantage by identifying individual microbial cells, crucial for slow-growing microbes and mixed infections.
  • High-speed FACS can expedite the characterization of challenging pathogens.
  • FACS provides a comprehensive approach, integrating pathogen identification with host immune response evaluation and therapeutic monitoring.