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Related Concept Videos

  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • A Gene Expression Signature Of Epithelial Tubulogenesis And A Role For Aspm In Pancreatic Tumor Progression.
  • Biomedical And Clinical Sciences
  • Oncology And Carcinogenesis
  • Predictive And Prognostic Markers
  • A Gene Expression Signature Of Epithelial Tubulogenesis And A Role For Aspm In Pancreatic Tumor Progression.

    • Related Experiment Videos

    A gene expression signature of epithelial tubulogenesis and a role for ASPM in pancreatic tumor progression.

    Wei-Yu Wang1, Chung-Chi Hsu, Ting-Yun Wang

    • 1Laboratory for Tumor Epigenetics and Stemness, National Institute of Cancer Research and Translational Center for Glandular Malignancies, National Health Research Institutes, Tainan, Taiwan; Department of Pathology, National Cheng-Kung University Hospital and College of Medicine, National Cheng Kung University, Tainan, Taiwan.

    Gastroenterology
    |July 31, 2013

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    A new gene signature linked to pancreatic epithelial tubulogenesis can predict pancreatic ductal adenocarcinoma (PDAC) patient outcomes after surgery, identifying those who may benefit from aggressive therapy.

    Keywords:
    3-dimensional3DASPMBiomarkerC-indexCSCDvlHPDEPDACPancreatic CancerPrognostic FactorTumor Progressionabnormal spindle-like microcephaly associatedcancer stem cellconcordance indexdishevelledhuman pancreatic ductal epithelialpancreatic ductal adenocarcinomarBMreconstituted basement membraneshRNAshort-hairpin RNA

    Related Experiment Videos

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genomics

    Background:

    • Pancreatic ductal adenocarcinoma (PDAC) frequently recurs or metastasizes post-surgery, necessitating predictive markers for aggressive treatment selection.
    • Tissue microarchitecture changes are early events in pancreatic tumorigenesis and are used in pathological grading.

    Purpose of the Study:

    • To investigate the association between gene expression changes during pancreatic epithelial morphogenesis and patient outcomes in PDAC.
    • To identify a gene expression profile that correlates with prognosis in surgically treated PDAC patients.

    Main Methods:

    • Generated 3D architectures of human pancreatic duct epithelial cells in a basement membrane matrix.
    • Identified gene expression profiles during tubulogenesis and PANC-1 spheroid formation.
    • Validated differential gene expression via immunoblot analysis and compared profiles with PDAC patient samples and outcomes.

    Main Results:

    • A tubulogenesis-associated gene expression profile, resembling differentiated pancreatic tissue, correlated with favorable patient outcomes.
    • A 6-28 gene tubulogenesis-specific signature accurately predicted PDAC prognosis in independent cohorts (81.2%-95.0% accuracy).
    • ASPM gene upregulation in PDAC correlated with poor outcomes and promoted aggressiveness by maintaining Wnt-β-catenin signaling and stem cell features.

    Conclusions:

    • Identified a gene expression profile linked to pancreatic epithelial tubulogenesis.
    • Developed a tissue architecture-specific signature for PDAC cells that predicts patient outcomes post-surgery.