Related Experiment Video

Updated: May 9, 2026

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

The receptor-like protein ReMAX of Arabidopsis detects the microbe-associated molecular pattern eMax from Xanthomonas

Anna Kristina Jehle1, Martin Lipschis, Markus Albert

  • 1Zentrum für Molekularbiologie der Pflanzen, University Tübingen, 72076 Tuebingen, Germany.

The Plant Cell
|July 31, 2013
PubMed

Insights

Scientists identified a plant immune receptor, ReMAX/RLP1, that detects the bacterial molecule eMax. This discovery advances understanding of plant-microbe interactions and offers potential for enhancing crop disease resistance.

Area of Science:

  • Plant immunity
  • Molecular biology
  • Microbial pathogenesis

Background:

  • Plants possess pattern recognition receptors (PRRs) to detect microbe-associated molecular patterns (MAMPs) as part of their immune defense.
  • The bacterial MAMP eMax from xanthomonads is recognized by specific PRRs in plants.

Purpose of the Study:

  • To identify the specific PRR in Arabidopsis thaliana responsible for detecting the bacterial MAMP eMax.
  • To investigate the genetic basis and functional characteristics of eMax perception.

Main Methods:

  • Genetic mapping of eMax perception in Arabidopsis thaliana accessions.
  • Functional complementation of receptor-like protein1 (RLP1) mutants using gene constructs.
  • Construction and testing of hybrid receptors for interfamily transfer.

Main Results:

  • A PRR, designated ReMAX (receptor of eMax), was identified in Arabidopsis thaliana with specificity for the bacterial MAMP eMax.
  • eMax perception was mapped to receptor-like protein1 (RLP1) and required a longer variant, ReMAX, for functional complementation.
  • Hybrid receptor technology enabled successful interfamily transfer of ReMAX function to Nicotiana benthamiana.

Conclusions:

  • ReMAX/RLP1 is the determinant of eMax specificity in plants.
  • Hybrid receptor technology is a viable strategy for enhancing PRR function and pathogen detection in crop plants.

More Related Videos

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana05:03

Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana

Published on: February 9, 2024

Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...