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Effects of artonin e on migration and invasion capabilities of human lung cancer cells
Kanok Plaibua1, Varisa Pongrakhananon, Preedakorn Chunhacha
1Departments of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phatumwan, Bangkok, Thailand 10330. pithi_chan@yahoo.com
Background:
Knowledge regarding substances that attenuate motility of cancer cells has gathered significant attention, as they benefit the development of novel anticancer strategies. The anti-migration and anti-invasion activities of artonin E, extracted from bark of Artocarpus gomezianus, were investigated in lung cancer cells in this study.
Materials And Methods:
Cytotoxicity and antiproliferative effects of artonin E were examined by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Migration and invasion assays were performed on H460, H23, A549 and H292 human lung cancer cells. Cell morphology was determined by phalloidin-rhodamine staining. Motility-related proteins were investigated by western blotting.
Results:
Artonin E exhibited anti-migration and anti-invasion activities in H460 cells. Cell morphology revealed that treatment of the cells with non-toxic concentrations of artonin E resulted in a decrease of activated focal adhesion kinase (FAK), downstream protein kinase B (AKT) activation, and Cell division cycle-42 (CDC42), all of which were associated with the anti-motility effect of this compound. Artonin E inhibited invasion and migration of other lung cancer cells, namely H292, H23 and A549 cells.
Conclusion:
These results suggest that artonin E may be a promising candidate for anti-metastasis use.
Insights
Artonin E, a compound from Artocarpus gomezianus, effectively inhibits lung cancer cell migration and invasion. This natural compound shows promise for developing new anti-metastasis cancer therapies.
Area of Science:
- Natural Product Chemistry
- Cancer Biology
- Pharmacology
Background:
- Substances that reduce cancer cell motility are crucial for developing novel anticancer strategies.
- Artonin E is a compound extracted from the bark of Artocarpus gomezianus.
- Its potential anti-migration and anti-invasion activities in lung cancer were unexplored.
Purpose of the Study:
- To investigate the anti-migration and anti-invasion activities of artonin E in human lung cancer cells.
- To explore the molecular mechanisms underlying artonin E's effect on cancer cell motility.
- To evaluate artonin E as a potential therapeutic agent against lung cancer metastasis.
Main Methods:
- Cytotoxicity and antiproliferative effects were assessed using the MTT assay.
- Migration and invasion assays were conducted on H460, H23, A549, and H292 lung cancer cell lines.
- Cell morphology was analyzed via phalloidin-rhodamine staining, and motility-related proteins were examined by western blotting.
Main Results:
- Artonin E demonstrated significant anti-migration and anti-invasion effects in H460 lung cancer cells.
- Non-toxic concentrations of artonin E reduced activated focal adhesion kinase (FAK), protein kinase B (AKT), and Cell division cycle-42 (CDC42) activation.
- Artonin E also inhibited migration and invasion in H292, H23, and A549 lung cancer cell lines.
Conclusions:
- Artonin E exhibits potent anti-metastasis properties against various human lung cancer cells.
- The compound's mechanism involves the downregulation of key motility-related proteins.
- Artonin E represents a promising candidate for the development of novel anti-metastasis therapies.
