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Updated: May 9, 2026
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
Published on: August 17, 2010
The effect of surgical wound on ovarian carcinoma growth in an animal model
Jeong-Won Lee1, Young-Ae Park, Young-Jae Cho
1Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-Dong, Gangnam-gu, Seoul, 135-710, Korea. garden.lee@samsung.com
Background/Aims:
We examined the effects according to the extent of surgical wound mimicking laparoscopy or laparotomy on ovarian cancer growth in an orthotopic mouse model.
Materials And Methods:
To mimic surgery effects, we performed laparoscopy or laparotomy on athymic nude mice under isoflurane inhalation at four days after tumor cell injection. For the laparoscopy model, we performed pneumoperitoneum with CO2, by inserting three cannulars.
Results:
Mice in the laparoscopy-mimicking group had significantly lower tumor weight compared to mice in the laparotomy group (p<0.05). In the immediate postoperative period, serum levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP2) were significantly lower in the laparoscopy group.
Conclusion:
These results indicate that a minimal surgical wound such as that on laparoscopy, appears to induce little surgical stress on enhancing tumor growth compared to laparotomy in an ovarian cancer animal model, possibly because it minimally influences the secretion of VEGF and MMP2.
