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Updated: May 9, 2026

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Interference from anti-streptavidin antibody.

Nichole Johnson Rulander¹, David Cardamone, Marilyn Senior

¹William Pepper Laboratories, Hospital of the University of Pennsylvania, Philadelphia, USA.

Archives of Pathology & Laboratory Medicine

|August 1, 2013

Summary

This summary is machine-generated.

This study identifies a novel immunoassay interference caused by endogenous anti-streptavidin antibodies. This interference can lead to inaccurate diagnostic results in clinical laboratory assays.

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Area of Science:

Clinical Chemistry
Immunology
Diagnostic Assays

Background:

Immunoassays are vital for clinical diagnosis but susceptible to interferences.
The streptavidin-biotin interaction is widely used in immunoassay platforms for its efficiency.
Potential interferences from endogenous antibodies are a concern for assay reliability.

Observation:

A patient with thyroid dysfunction exhibited abnormal T-uptake and nonlinear dilution of thyroid stimulating hormone.
These results suggested a previously undocumented immunoassay interference.
Pretreatment with Protein-A sepharose corrected the nonlinear dilution, indicating interference.

Findings:

The study describes the first documented case of endogenous anti-streptavidin antibodies interfering with clinical laboratory assays.
Interference patterns included falsely decreased results in sandwich assays and falsely elevated results in competitive assays.
Streptavidin-agarose adsorption yielded results comparable to Protein-A sepharose, confirming the interference source.

Implications:

This discovery highlights a new source of interference affecting multiple immunoassay platforms.
Awareness of anti-streptavidin antibodies is crucial for accurate interpretation of immunoassay results.
Further research is needed to develop strategies for mitigating this interference in diagnostic testing.