Chromium reduction in Pseudomonas putida
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Y Ishibashi1, C Cervantes, S Silver
1Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.
Applied and Environmental Microbiology
|July 1, 1990
Abstract:
Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.