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Related Experiment Video

Updated: May 9, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

A novel dynamic flush method to reduce column-related carryover.

Susan Chen¹, Ji Zhang¹, Debra Liao¹

¹Department of Drug Metabolism and Pharmacokinetics, Millennium Pharmaceuticals, Inc, 40 Landsdowne St., Cambridge, MA 02139, USA.

Journal of Chromatographic Science

|August 1, 2013

Summary

A new dynamic flush method reverses liquid chromatography column flow to reduce carryover by up to 94.4% for "sticky" compounds. This technique improves accuracy and precision in liquid chromatography-tandem mass spectrometry (LC-MS/MS) without compromising performance.

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Curtain Flow Column: Optimization of Efficiency and Sensitivity

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

Area of Science:

Analytical Chemistry
Chromatography

Background:

Column-related carryover significantly impacts accuracy and precision in LC-MS/MS.
Persistent carryover is a challenge, especially for
sticky
compounds.

Purpose of the Study:

To develop a straightforward dynamic flush method to reduce column-related carryover.
To evaluate the effectiveness of the method in minimizing carryover without additional blank runs.

Main Methods:

A novel dynamic flush method was developed using a Valco switching valve and instrument control software.
The method involves alternating column flow direction, switching the contaminated inlet to the outlet for flushing during the next run.

Main Results:

Column-related carryover for tested
sticky
compounds was reduced by 52.3-94.4% compared to non-dynamic flush methods.
No compromise in HPLC separation performance (retention time, peak shape) or reproducibility was observed after over 300 injections.

Conclusions:

The dynamic flush method effectively reduces column-related carryover in LC-MS/MS analysis.
This simple and implementable technique enhances analytical accuracy and precision for challenging compounds.