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Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

TRP53 activates a global autophagy program to promote tumor suppression

Daniela Kenzelmann Broz1, Laura D Attardi

  • 1Division of Radiation and Cancer Biology; Department of Radiation Oncology; Stanford University School of Medicine; Stanford, CA USA.

Autophagy
|August 1, 2013
PubMed

Insights

The tumor suppressor TP53 (tumor protein 53) regulates autophagy, a cellular process. This autophagy program is crucial for TP53-mediated apoptosis and tumor suppression following DNA damage.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The tumor suppressor function of the TP53 (tumor protein 53) transcription factor is critical for preventing cancer.
  • However, the precise molecular mechanisms underlying TP53's tumor suppressor activity are not fully elucidated.

Purpose of the Study:

  • To investigate the global transcriptional networks regulated by TP53 in response to DNA damage.
  • To identify novel pathways and functions associated with TP53 in cellular processes.

Main Methods:

  • Utilized ChIP-seq (Chromatin Immunoprecipitation sequencing) to map TP53 binding sites genome-wide.
  • Employed RNA-seq (RNA sequencing) to analyze changes in gene expression profiles.
  • Studied primary cells subjected to DNA damage to mimic cellular stress conditions.

Main Results:

  • Identified a significant TP53-regulated autophagy program.
  • Demonstrated that TP53 family members, TP63 and TP73, can also regulate this autophagy pathway.
  • Found that autophagy is not involved in TP53-dependent cell cycle arrest but contributes to apoptosis and tumor suppression.

Conclusions:

  • TP53 plays a profound role in regulating autophagy through an extensive transcriptional network.
  • The identified autophagy program is essential for TP53-mediated apoptosis and tumor suppression.
  • These findings provide new insights into TP53 biology and its function as a tumor suppressor.
Keywords:
ChIP-seqRNA-seqapoptosisautophagyp53tumor suppression

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