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Updated: May 9, 2026
High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Evaluation of hot compressed water pretreatment and enzymatic saccharification of tulip tree sawdust using severity
Dae Sung Kim1, Aye Aye Myint, Hun Wook Lee
1School of Chemical and Biological Engineering and Institute of Chemical Processes, Seoul National University, Seoul, Republic of Korea.
Abstract:
Tulip tree sawdust was pretreated using hot compressed water with different pretreatment severities (LogR0, 3.05-5.01) by varying reaction temperatures (180-220°C) and residence time (1-30 min). It is found that the chemical composition and physicochemical properties of the pretreated products can be characterized and correlated with severity. Removal of most of the xylan and other hemicellulosic sugars from the raw material was observed at a severity of 4.5. Thus, the residual solids were recovered with increased cellulose and lignin contents. Nearly complete glucan conversion was achieved after 48 h of hydrolysis with 10 FPU/g of wet residual solid obtained above a severity of 4.8. The characteristics of the pretreated solids according to the pretreatment severity were strongly related with the glucose yield. The removal of structural barriers to the enzyme attack was the dominant factor affecting enzyme accessibility to the substrate.
