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Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Published on: July 21, 2020
Characterization of human upper airway epithelial progenitors
Dawn T Bravo1, Ethan Soudry, Justin A Edward
1Department of Otolaryngology, Stanford University School of Medicine, Stanford, CA.
International Forum of Allergy & Rhinology
|August 1, 2013
Summary
Researchers identified rare human nasal epithelial progenitor cells using surface markers nerve growth factor receptor (NGFR) and intercellular adhesion molecule-1 (ICAM1). These cells can regenerate airway epithelium, offering potential for treating upper airway diseases.
Area of Science:
- * Regenerative Medicine
- * Epithelial Biology
- * Stem Cell Research
Background:
- * Nasal epithelial cells regenerate via progenitor cells.
- * Upper airway diseases can impair progenitor cell function.
- * This study defines rare human nasal epithelial progenitor cells.
Purpose of the Study:
- * To identify and characterize rare human nasal epithelial progenitor cells.
- * To establish markers for detecting these progenitor cells.
- * To assess the regenerative potential of identified progenitor cells.
Main Methods:
- * Human ethmoid sinus tissue processed into single-cell suspensions.
- * High-dimensional flow cytometry used with antibodies against EpCAM, CD45, NGFR, ICAM1, and ITGA6.
- * Fluorescence minus one (FMO) controls applied for accurate gating.
Main Results:
- * A rare cell population (approx. 0.06%) identified as EpCAM(-) CD45(-) NGFR(+) ICAM1(+).
- * Purified lineage-negative cells uniformly expressed p63.
- * In vitro culture demonstrated spontaneous differentiation into epithelial cells.
Conclusions:
- * Identified NGFR(+) ICAM1(+) cells as promising human nasal epithelial progenitors.
- * These cells hold potential for upper airway regeneration.
- * Further investigation is warranted for their therapeutic applications.
