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Updated: May 9, 2026
07:50
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Basic transcription factor 3 is involved in gastric cancer development and progression
Qi Liu1, Jian-Ping Zhou, Bin Li
1Department of Anorectal Surgery, People's Hospital of Hunan Province, First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan Province, China.
World Journal of Gastroenterology
|August 1, 2013
Summary
Basic transcription factor 3 (BTF3) is upregulated in gastric tumors, promoting proliferation and inhibiting apoptosis. Silencing BTF3 in gastric cancer cells reduced proliferation and colony formation, suggesting BTF3 as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Gene Expression
Background:
- Basic transcription factor 3 (BTF3) is implicated in cancer development.
- Previous findings indicated BTF3 upregulation in gastric tumor samples.
Purpose of the Study:
- To investigate the role of BTF3 in gastric cancer.
- To analyze the functional impact of BTF3 on gastric cancer cell proliferation, cell cycle, and apoptosis.
Main Methods:
- Quantitative real-time PCR analyzed BTF3 mRNA levels in tumor and normal gastric tissues and cell lines.
- Stable BTF3 silencing was achieved using lentivirus-mediated siRNA in SGC-7901 cells.
- Cell proliferation, cell cycle, apoptosis, and colony formation were assessed using various assays.
Main Results:
- BTF3 mRNA expression was significantly higher in gastric tumors than in normal tissues and elevated in all tested cancer cell lines.
- Silencing BTF3 in SGC-7901 cells led to decreased proliferation, reduced G1 phase arrest, increased S and G2/M phase percentages, diminished colony formation, and increased apoptosis.
Conclusions:
- BTF3 expression is correlated with enhanced proliferation and impaired apoptosis in gastric cancer.
- BTF3 silencing effectively suppresses gastric cancer cell proliferation and colony formation, highlighting its potential as a therapeutic target.
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