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[Study on ultrasonic extraction and macroporous resin purification techniques of total flavones from Sedum lineare]
Jian-Wei Su1, Zhi-Min Jia, Yang Xie
1School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. sujianwei-202@163.com
Objective:
To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin.
Methods:
Optimum ultrasonic extraction conditions (ethanol concentration, solid/liquid, ultrasonic time, working temperature) were obtained through orthogonal design. The adsorption ratio and eluting ratio of total flavones were selected as indexes.
Results:
The optimum ultrasonic extraction condition was 80% ethanol, solid/liquid 1:40, ultrasonic 20 min at 30 degrees C. The adsorbed AB-8 macroporous resin column was eluted by 80% ethanol at the eluting velocity of 2 mL/min, the eluting ratio and purity of total flavones was 94.72% and 43.51%, respectively.
Conclusion:
The method is simple and low cost.