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04:53
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Rhodamine cyclic hydrazide as a fluorescent probe for the detection of hydroxyl radicals
Minjeong Kim1, Sung-Kyun Ko, Hyemi Kim
1Department of Chemistry, Yonsei University, Seoul 120-749, Korea. jstae@yonsei.ac.kr injae@yonsei.ac.kr.
Chemical Communications (Cambridge, England)
|August 2, 2013
Abstract:
A new rhodamine fluorescent probe for monitoring ˙OH has been developed based on the oxidative C-H abstraction reaction of rhodamine cyclic hydrazide. The probe exhibits excellent selectivity for ˙OH with virtually no interference by other ROS/RNS species. Fluorescent imaging of A549 and RAW264.7 cells is also successfully demonstrated to detect intracellular ˙OH in live cells.
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