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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

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Published on: April 9, 2012

PCR as a diagnostic tool for extra-pulmonary tuberculosis.

Ganavalli S Ajantha¹, Praveen C Shetty, Raghavendra D Kulkarni

¹Professor, Department of Microbiology, SDM College of Medical Sciences & Hospital , Dharwad-580009, Karnataka, India .

Journal of Clinical and Diagnostic Research : JCDR

|August 2, 2013

Summary

Polymerase Chain Reaction (PCR) significantly improves the detection of Extra-Pulmonary Tuberculosis (EPTB) when combined with conventional methods. Including PCR in diagnostic panels enhances laboratory identification of Mycobacterium tuberculosis.

Keywords:

Extrapulmonary tuberculosis Mycobacterium PCR Tuberculosis

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Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

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Published on: February 16, 2020

Area of Science:

Microbiology
Infectious Diseases
Molecular Diagnostics

Background:

Extra-Pulmonary Tuberculosis (EPTB) constitutes about 40% of all tuberculosis cases, contributing significantly to morbidity.
While not communicable, EPTB diagnosis presents challenges, necessitating advanced diagnostic tools.
This study evaluated the utility of Polymerase Chain Reaction (PCR) in diagnosing EPTB.

Purpose of the Study:

To assess the efficacy of Polymerase Chain Reaction (PCR) as an adjunct diagnostic tool for Extra-Pulmonary Tuberculosis (EPTB).
To compare the diagnostic yield of PCR against conventional methods like Ziehl-Neelsen staining and Lowenstein-Jensen culture.
To determine the added value of molecular testing in the laboratory diagnosis of EPTB.

Main Methods:

Clinical samples from suspected EPTB cases were analyzed.
Conventional diagnostic methods included Ziehl-Neelsen staining (ZNS) and culture on Lowenstein-Jensen medium (LJM).
Polymerase Chain Reaction (PCR) testing was performed using a commercial kit on homogenized samples.

Main Results:

Out of 182 samples tested, 22 were positive by at least one method.
Polymerase Chain Reaction (PCR) detected the highest number of EPTB cases, followed by culture.
Statistical analysis using McNemar's test indicated a significant improvement in detection rates when using PCR.

Conclusions:

Combining available diagnostic tests, including PCR, increases laboratory detection rates for Mycobacterium tuberculosis in clinical samples.
Polymerase Chain Reaction (PCR) should be integrated into the standard diagnostic panel for Extra-Pulmonary Tuberculosis (EPTB).
Enhanced diagnostic capabilities for EPTB can lead to earlier and more accurate patient management.