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Related Concept Videos

Mixing Time01:19

Mixing Time

The concept of mixing time is significant in producing a uniform concrete mix with the required strength. The mixing period starts once all components are in the mixer. Initially, the mixer is charged with 10% of the water, followed by the consistent addition of solids and then 80% of the water. The remaining water is added later, within the first quarter of the mixing period. The minimum mixing time varies according to the mixer's capacity; for example, mixers with up to 1 cubic yard capacity...
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Framing Effects

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Bacterial generation time, the period required for a bacterial population to double during its exponential growth phase, serves as a critical measure of microbial growth dynamics under optimal conditions. This parameter varies significantly across bacterial species and can be influenced by factors such as temperature, pH, and the availability of nutrients. For example, Escherichia coli can achieve a generation time of approximately 20 minutes, while Mycobacterium tuberculosis exhibits a much...
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation
06:53

Creating Virtual-hand and Virtual-face Illusions to Investigate Self-representation

Published on: March 1, 2017

"Making it real" time.

Kent E Vrana1

  • 1Department of Pharmacology, Penn State College of Medicine, PA, USA.

Biotechniques
|August 2, 2013
PubMed
Summary
This summary is machine-generated.

Polymerase chain reaction (PCR) has advanced significantly over 30 years. Real-time monitoring of DNA amplification was revolutionized by SYBR Green dye, a key development reported in 1997.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • The polymerase chain reaction (PCR) technique, first described 30 years ago, has undergone substantial advancements.
  • Real-time monitoring of PCR amplification is crucial for understanding DNA amplification dynamics.

Purpose of the Study:

  • To highlight the historical significance of real-time PCR monitoring.
  • To recognize the impact of SYBR Green dye in continuous DNA amplification monitoring.

Main Methods:

  • Review of seminal reports in the field of PCR.
  • Focus on the development and application of real-time monitoring techniques.

Main Results:

  • The development of real-time monitoring paradigms transformed PCR applications.
  • SYBR Green dye emerged as a pivotal tool for continuous DNA amplification assessment.

Conclusions:

  • SYBR Green dye, introduced in 1997, became a standard for real-time PCR.
  • The evolution of PCR monitoring techniques has been central to its widespread success.