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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.

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A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
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A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)

Published on: July 11, 2017

Technical aspects of inductively coupled plasma bioanalysis techniques.

Jennifer Ammerman1, Chaoyang Huang, Jeffrey Sailstad

  • 1MPI Research, 54943 N. Main Street, Mattawan, MI 49071, USA. jennifer.ammerman@ mpiresearch.com

Bioanalysis
|August 3, 2013
PubMed
Summary

This paper discusses quantifying inorganic elements in biological samples for pharmaceutical studies using inductively coupled plasma (ICP) techniques. It highlights challenges with current regulations and endogenous elements impacting accurate quantification.

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A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
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Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis
  • Bioanalysis

Background:

  • Inductively Coupled Plasma (ICP) techniques have a long history in environmental analysis and are increasingly used in the pharmaceutical industry.
  • Existing bioanalytical method validation and sample analysis regulatory guidance are primarily designed for chromatographic methods and may not fully address ICP techniques.
  • Quantifying inorganic elements in biomatrices is crucial for pharmaceutical development, including nonclinical and clinical studies.

Purpose of the Study:

  • To address the technical aspects of quantifying pharmaceutically derived inorganic elements in biomatrices.
  • To support Good Laboratory Practice (GLP) nonclinical and clinical studies.
  • To provide insights into the application of ICP techniques in pharmaceutical bioanalysis.

Main Methods:

  • Focus on inductively coupled plasma mass spectrometry (ICP-MS), including quadrupole and high-resolution ICP-MS.
  • Discusses the quantification of inorganic elements in pharmaceutical compounds and biological matrices.
  • Highlights the importance of method validation and sample analysis for ICP techniques.

Main Results:

  • ICP techniques, particularly ICP-MS, are increasingly employed for quantifying inorganic elements in pharmaceutical contexts.
  • Current regulatory guidance for bioanalytical methods may not be fully applicable to all aspects of ICP techniques.
  • Endogenous elements present in biomatrices can significantly impact the quantification of blanks, standard curves, quality control samples, and the lower limit of quantification (LLOQ).

Conclusions:

  • There is a need for tailored validation and regulatory considerations for ICP techniques in pharmaceutical bioanalysis.
  • Understanding and managing the impact of endogenous elements is critical for accurate inorganic element quantification in biomatrices.
  • The application of ICP-MS offers advanced capabilities for elemental analysis in pharmaceutical research and development.