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Related Concept Videos

Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Rab Cascades01:25

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins, also known...
GTPases and their Regulation02:14

GTPases and their Regulation

Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins, also known...
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...

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Related Experiment Video

Updated: May 9, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
11:28

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates

Published on: March 31, 2012

Rab35: GEFs, GAPs and effectors.

Mathilde Chaineau1, Maria S Ioannou, Peter S McPherson

  • 1Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec, H3A 2B4, Canada.

Traffic (Copenhagen, Denmark)
|August 3, 2013
PubMed
Summary

Rab35, a key regulator of endosomal recycling and actin cytoskeleton, is controlled by specific GEFs and GTPase-activating proteins. Its function links Rab and Arf GTPases, impacting tumor formation.

Keywords:
ArfDENN domainDENNDGAPGEFGTPaseRabRab35TBC domainclathrin-coated vesicleendosome

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Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

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Last Updated: May 9, 2026

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Rabs are essential small GTPases regulating membrane trafficking.
  • Over 60 Rabs exist in humans, with Rab35 recently identified as crucial for endosomal cargo recycling and actin cytoskeleton regulation.

Purpose of the Study:

  • To investigate the regulation of Rab35 activity by specific guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
  • To explore the functional insights gained from analyzing Rab35 effectors.
  • To elucidate the novel link between Rab35 and Arf GTPases and its implications in cancer.

Main Methods:

  • Focus on the connecdenn/DENND1 family of GEFs and the TBC1D10/EPI64 family of GAPs in regulating Rab35.
  • Analysis of Rab35 effector proteins.
  • Investigating the interplay between Rab and Arf GTPase families.

Main Results:

  • Detailed examination of Rab35 regulation by specific GEFs and GAPs.
  • Identification of numerous Rab35 effectors providing functional insights.
  • Established Rab35 as a link between Rab and Arf GTPases.

Conclusions:

  • Rab35 activity is precisely controlled by connecdenn/DENND1 GEFs and TBC1D10/EPI64 GAPs.
  • Rab35 plays a significant role in membrane trafficking and actin dynamics.
  • Rab35 links Rab and Arf GTPases, with implications for tumor biology and invasiveness.