Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

Septins

Septins

Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dissociation of the nuclear basket triggers chromosome loss in aging yeast.

eLife·2025

Same author

A network of P-body and Whi3 condensates adjusts cell fate decisions to cellular context.

Molecular cell·2025

Same author

The Yin and Yang of replicative aging and rejuvenation.

Current opinion in genetics & development·2025

Same author

Global profiling of protein complex dynamics with an experimental library of protein interaction markers.

Nature biotechnology·2024

Same author

Correction: Fission-independent compartmentalization of mitochondria during budding yeast cell division.

The Journal of cell biology·2024

Same author

Microtubule specialization by +TIP networks: from mechanisms to functional implications.

Trends in biochemical sciences·2024

Same journal

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026

Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026

Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026

Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026

Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026

Same journal

The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair.

Developmental cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 9, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Bud building by septin patch hole punching.

Fabrice Caudron¹, Yves Barral

¹Department of Biology, Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, 8093 Zurich, Switzerland.

Developmental Cell

|August 3, 2013

Summary

This summary is machine-generated.

Small GTPase Cdc42 initiates cell polarity in yeast. A new study reveals Cdc42, septins, and exocytosis form a feedback system to maintain this polarity site.

More Related Videos

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Published on: July 2, 2012

Related Experiment Videos

Last Updated: May 9, 2026

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Published on: August 17, 2022

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Published on: July 2, 2012

Area of Science:

Cell Biology
Molecular Biology
Systems Biology

Background:

Small GTPase Cdc42 is crucial for establishing cell polarity in budding yeast.
Polarity establishment involves complex regulatory networks.

Purpose of the Study:

To investigate the integrated roles of Cdc42, septins, and the exocytosis pathway in polarity site definition and insulation.
To elucidate the feedback mechanisms governing yeast cell polarity.

Main Methods:

In silico modeling to simulate cellular processes.
Experimental cell biology techniques to validate model predictions.

Main Results:

Cdc42, septins, and the exocytosis pathway are integrated into a cohesive feedback system.
This system effectively defines and insulates the site of polarity on the cell membrane.

Conclusions:

The interplay between Cdc42, septins, and exocytosis is essential for robust polarity establishment in yeast.
Feedback regulation ensures the precise localization and maintenance of the polarity site.