Related Experiment Video
Updated: May 9, 2026

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
A replicase of Potato virus X acts as the resistance-breaking determinant for JAX1-mediated resistance
Kyoko Sugawara1, Takuya Shiraishi, Tetsuya Yoshida
1Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Abstract:
Lectin-mediated resistance (LMR) has been suggested to comprise an uncharacterized branch of antiviral plant innate immunity. To unveil the feature of resistance conferred by jacalin-type lectin required for potexvirus resistance 1 (JAX1), a recently isolated LMR gene against potexviruses, we analyzed the resistance-breaking variants to find the viral component involved in resistance. We employed grafting-mediated inoculation, a high-pressure virus inoculation method, to obtain Potato virus X (PVX) variants that can overcome JAX1-mediated resistance. Whole-genome sequencing of the variants suggested that a single amino acid in the methyl transferase domain of the replicase encoded by PVX is responsible for this resistance-breaking property. Reintroduction of the amino-acid substitution to avirulent wild-type PVX was sufficient to overcome the JAX1-mediated resistance. These results suggest that viral replicase is involved in JAX1-mediated resistance. The residue that determines the resistance-breaking properties was highly conserved among potexviruses, suggesting a general role of the residue in potexvirus-JAX1 interactions.
Related Concept Videos
The JAK-STAT Signaling Pathway
Subviral Agents
Inhibitors of Viral Protein Synthesis
Restarting Stalled Replication Forks
Viral Mutations
Leaky Scanning

