Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are typically...

Glycosaminoglycans

Glycosaminoglycans

Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanisms of Resistance, Insights From Case Reports, and Future Prospects in Mycobacteriophage Therapy.

Journal of the Royal Society of New Zealand·2026

Same author

Nurr1 deficiency orchestrates a coupled liver-gut pathological axis revealed by multi-omics and deep-learning histopathology.

Frontiers in immunology·2026

Same author

Fenchone and cinnamyl acetate attenuate L-NAME-induced hypertension via modulation of vascular dysfunction, oxidative stress, and renal injury: An integrated network pharmacology, molecular docking, and ADMET study.

European journal of pharmacology·2026

Same author

Investigating the phytotherapeutic efficacy of <i>Acacia nilotica</i> pod ethanolic extract in modulating lipid profile, oxidative stress and inflammation in diet-induced hypercholesterolemia: <i>in vivo</i> and <i>in silico</i> insights.

Frontiers in pharmacology·2026

Same author

EACCO: Optimizing the Computation and Communication in Resource-Constrained IoT Devices for Energy-Efficient Swarm Robotics.

Sensors (Basel, Switzerland)·2026

Same author

Migraine, headache disorders, and brain health: aligning advocacy with the UN Sustainable Development Goals.

The journal of headache and pain·2026

Search research articles

Related Experiment Video

Updated: May 9, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

New acylated oligosaccharides from Pistacia integerrima.

Zia Ullah¹, Rashad Mehmood, Muhammad Imran

¹a Department of Chemistry , University of Karachi , Karachi , 75270 , Pakistan.

Natural Product Research

|August 3, 2013

Summary

Two novel acylated oligosaccharides, Integrisides A and B, were identified in Pistacia integerrima. Their chemical structures were determined using advanced spectroscopic methods and hydrolysis.

More Related Videos

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Related Experiment Videos

Last Updated: May 9, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Published on: September 20, 2016

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Area of Science:

Natural Product Chemistry
Phytochemistry
Organic Chemistry

Background:

Pistacia integerrima is a plant species with a history of traditional medicinal use.
The chemical constituents of P. integerrima are not fully characterized, presenting opportunities for novel compound discovery.

Purpose of the Study:

To isolate and characterize new chemical entities from Pistacia integerrima.
To elucidate the structures of novel acylated oligosaccharides.

Main Methods:

Extraction of aerial parts of Pistacia integerrima using methanol.
Fractionation of the methanolic extract, focusing on the n-butanol-soluble sub-fraction.
Isolation of compounds using chromatographic techniques.
Structure elucidation employing spectroscopic methods (e.g., NMR, MS) and chemical hydrolysis.

Main Results:

Two new acylated oligosaccharides, designated Integriside A (1) and Integriside B (2), were successfully isolated.
The structures of Integrisides A and B were fully determined through comprehensive spectroscopic analysis and hydrolysis data.

Conclusions:

The study successfully identified and characterized two novel acylated oligosaccharides from Pistacia integerrima.
The findings contribute to the phytochemical knowledge of Pistacia integerrima and introduce new molecular structures for potential further investigation.