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Updated: May 9, 2026
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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Published on: September 2, 2014
Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots
Melanie Krebs1, Karin Schumacher
1Department of Developmental Biology, Centre for Organismal Studies, University of Heidelberg, 69120 Heidelberg, Germany. melanie.krebs@cos.uni-heidelberg.de
Cold Spring Harbor Protocols
|August 3, 2013
Abstract:
Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators.
