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Related Concept Videos

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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S-Cdk Initiates DNA Replication

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Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

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Updated: May 9, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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Is PCNA unloading the central function of the Elg1/ATAD5 replication factor C-like complex?

Takashi Kubota1, Kyungjae Myung, Anne D Donaldson

  • 1Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK.

Cell Cycle (Georgetown, Tex.)
|August 3, 2013
PubMed
Summary

The Elg1/ATAD5-replication factor C-like complex (RLC) unloads the PCNA protein from DNA after replication. This function is vital for maintaining genome stability in yeast and human cells, preventing genomic instability and tumorigenesis.

Keywords:
ATAD5Elg1PCNAPCNA unloadingRFCgenome stability

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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Genome stability is essential for cellular function and preventing diseases like cancer.
  • The Elg1 protein in yeast and its human homolog ATAD5 are crucial for genome maintenance.
  • The Elg1/ATAD5-replication factor C-like complex (RLC) interacts with PCNA but its function was unclear.

Purpose of the Study:

  • To elucidate the molecular function of the Elg1/ATAD5-RLC.
  • To understand the role of Elg1/ATAD5-RLC in PCNA regulation.
  • To connect PCNA unloading to genome stability.

Main Methods:

  • Review of recent studies in yeast and human cell models.
  • Analysis of protein-protein interactions between Elg1/ATAD5-RLC and PCNA.
  • Examination of cellular phenotypes associated with Elg1/ATAD5 deficiency.

Main Results:

  • Elg1/ATAD5-RLC is identified as a PCNA (proliferating cell nuclear antigen) unloader.
  • PCNA removal from chromatin post-replication is mediated by Elg1/ATAD5-RLC.
  • Loss of Elg1/ATAD5 function leads to increased genomic instability.

Conclusions:

  • Elg1/ATAD5-RLC plays a critical role in removing PCNA from chromatin after DNA replication.
  • PCNA unloading by Elg1/ATAD5-RLC is a key mechanism for maintaining genome stability.
  • Further research is needed to fully understand the link between PCNA unloading and Elg1/ATAD5's role in preventing tumorigenesis.