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TIS21/BTG2 inhibits invadopodia formation by downregulating reactive oxygen species level in MDA-MB-231 cells

Jung-A Choi1, In Kyoung Lim

  • 1Department of Biochemistry and Molecular Biology, Brain Korea 21 Division of Cell Transformation and Restoration, Ajou University School of Medicine, Suwon, 443-721, Korea.

Journal of Cancer Research and Clinical Oncology
|August 3, 2013
PubMed
Abstract

Insights

The tumor suppressor TIS21 inhibits breast cancer cell invasion by suppressing invadopodia formation and reducing reactive oxygen species (ROS). This finding offers new insights into controlling cancer cell metastasis.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Cancer cell invasion relies on invadopodia, which degrade the extracellular matrix.
  • The precise mechanisms governing invadopodia formation in cancer cells are not fully understood.

Purpose of the Study:

  • To investigate the role of TIS21 in regulating invadopodia formation and cancer cell invasion.
  • To explore the involvement of reactive oxygen species (ROS) in TIS21-mediated invasion suppression.

Main Methods:

  • Adenoviral transduction of breast cancer cells with Ad-LacZ or Ad-TIS21.
  • Assessment of invasion activity using invadopodia and Matrigel assays.
  • Monitoring intracellular ROS levels via FACS-based analysis.

Main Results:

  • TIS21 significantly suppressed invadopodia formation, invasion, and F-actin remodeling.
  • TIS21 expression led to reduced ROS generation, crucial for invadopodia formation.
  • These effects were confirmed in TIS21-deficient mouse embryonic fibroblast (MEF) cells.

Conclusions:

  • TIS21 acts as a suppressor of invadopodia formation and cancer cell invasion.
  • TIS21-mediated inhibition of ROS is a key mechanism regulating invadopodia formation.
  • This study provides novel insights into invasion signaling pathways involving the tumor suppressor TIS21 in breast cancer.

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