Related Experiment Video
Updated: May 9, 2026

Dissection of Drosophila Ovaries
Published on: October 19, 2006
Control of e2f1 and PCNA by Drosophila transcription factor DREF
Akihito Kawamori1, Kouhei Shimaji, Masamitsu Yamaguchi
1Department of Applied Biology and Insect Biomedical Research Center, Kyoto Institute of Technology, Sakyo-ku, Kyoto, Japan.
Abstract:
DREF (DNA replication-related element-binding factor), a zinc finger type transcription factor required for proper cell cycle progression in both mitotic and endocycling cells, is a positive regulator of E2F1, an important transcription factor which regulates genes related to the S-phase of the cell cycle. DREF and E2F1 regulate similar sets of replication-related genes, including proliferating cell nuclear antigen (PCNA), and play roles in the G1 to S phase transition. However, the relationships between dref and e2f1 or PCNA during development are poorly understood. Here, we provided evidence for novel control of e2f1 and PCNA involving DREF in endocycling cells. Somatic clone analysis demonstrated that dref knockdown stabilized E2F1 expression at posttranscriptional levels in endocycling salivary gland cells. Similarly, PCNA expression was up-regulated in the endocycling salivary gland cells. Genetic interaction analysis indicated that the endoreplication defects are partly caused via possible enhancement of E2F1 activity. From these results and previous reports, we conclude that regulation of e2f1 and PCNA by DREF in vivo is complex and the regulation mechanism may differ with the tissue and/or positions in the tissue.
Related Concept Videos
RNA Polymerase II Accessory Proteins
Negative Regulator Molecules
Master Transcription Regulators
Circadian Rhythms and Gene Regulation
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Position-effect Variegation

