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Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis
Published on: March 17, 2023
Hemostasis disorders caused by polymer coated iron oxide nanoparticles
Lamiaa M A Ali1, Martín Gutiérrez, Rosa Cornudella
1instituto de Ciencia de Materiales de Aragón, CSIC - Universidad de Zaragoza, and Departamento de Física de la Materia Condensada, Facultad de Ciencias, 50009 Zaragoza, Spain.
Journal of Biomedical Nanotechnology
|August 6, 2013
Summary
Superparamagnetic iron oxide nanoparticles (SPIONs) coatings, specifically poly(4-vinyl pyridine)-graft-polyethylene glycol (P4VP-g-PEG), show anticoagulant effects by prolonging activated partial thromboplastin time (aPTT). These bioferrofluids do not impact complete blood counts or cause hemolysis.
Area of Science:
- Biomaterials Science
- Nanomedicine
- Hematology
Background:
- Superparamagnetic iron oxide nanoparticles (SPIONs) are crucial nanomaterials for biomedical applications.
- Polymeric coatings are essential for creating stable, biocompatible bioferrofluids from SPIONs.
- Evaluating the hemostatic behavior of SPION-based bioferrofluids is critical for safe intravenous administration.
Purpose of the Study:
- To assess the hemocompatibility of polymer-coated bioferrofluids and their components.
- To investigate the impact of bioferrofluids on blood coagulation parameters (PT, aPTT).
- To evaluate effects on complete blood count (CBC) and hemolysis.
Main Methods:
- A specific SPIONs/bioferrofluid model using poly(4-vinyl pyridine) (P4VP) and polyethylene glycol (PEG) was utilized.
- Blood coagulation was assessed by measuring prothrombin time (PT) and activated partial thromboplastin time (aPTT).
- Complete blood count (CBC) and in vitro hemolysis were analyzed.
Main Results:
- Increased bioferrofluid concentrations significantly prolonged activated partial thromboplastin time (aPTT), indicating inhibition of the intrinsic coagulation pathway.
- Prothrombin time (PT) remained unaffected.
- Polyethylene glycol (PEG) alone had no effect on coagulation, while the P4VP-g-PEG copolymer exhibited a strong anticoagulant effect, attributed to P4VP.
Conclusions:
- The P4VP component of the P4VP-g-PEG coating is responsible for the observed anticoagulant properties of the bioferrofluids.
- The studied bioferrofluids demonstrated no adverse effects on complete blood counts or in vitro hemolysis.
- These findings are important for the clinical translation of SPION-based bioferrofluids.
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