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Updated: May 9, 2026
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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
Published on: February 12, 2022
Transplantable liver organoids made from only three ingredients
Holger Willenbring1, Alejandro Soto-Gutierrez
1Department of Surgery, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, and Liver Center, University of California, San Francisco, San Francisco, CA 94143, USA. willenbringh@stemcell.ucsf.edu
Cell Stem Cell
|August 6, 2013
Summary
Induced pluripotent stem cells (iPSCs) are being developed for liver cell therapy. A new study shows iPSC-derived hepatocytes combined with other cells form organoids that survive and function outside the liver, expanding treatment possibilities.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Hepatology
Background:
- Induced pluripotent stem cells (iPSCs) offer potential for liver cell therapies.
- Current limitations exist in the application of iPSC-derived hepatocytes for treating diverse liver diseases.
Purpose of the Study:
- To investigate the potential of iPSC-derived hepatocytes in treating a broader range of liver diseases.
- To develop functional liver organoids capable of extrahepatic survival and function.
Main Methods:
- Generation of liver organoids by combining induced pluripotent stem cell-derived hepatocytes with endothelial and stromal cells.
- Assessment of organoid survival and function in an extrahepatic environment.
Main Results:
- The developed organoids demonstrated successful survival and functionality outside the liver.
- This approach expands the potential applications of iPSC-based liver therapies.
Conclusions:
- Combining iPSC-derived hepatocytes with endothelial and stromal cells creates functional organoids.
- These organoids show promise for treating a wider spectrum of liver diseases through extrahepatic engraftment and function.
