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Updated: Aug 10, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Abstract:
The cbh1 strong promoter was employed to over-express the cbh2 gene for enhancing cellobiohydrolase (CBH) production in Trichoderma reesei because CBH II component has higher specific activity than CBH I and is an important component in cellulase. The recombinant plasmid pCAMBIA1300-hph-PsCT containing strong expression cassette was constructed and transformed into T. reesei via optimized Agrobacterium-mediated transformation, producing 324 positive T. reesei transformants for the two steps of screening. Ten fast-growing T. reesei transformants were selected, amongst which C10 was found to have the highest filter paper activity 28.92±2.45 IU/mL, 4.3-fold higher than that of ZU-02, 6.71±0.79 IU/mL. C10 also has the highest cellobiohydrolase activity 122.44±7.42 U/mL, 5.4 times higher than that of ZU-02, 22.49±2.27 U/mL. The cellulase from C10 performed better (93.06±2.83%) than the one from ZU-02 in enzymatic hydrolysis because the exo-exo-synergism played a role.
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