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Updated: May 9, 2026
13:57
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
Understanding allosteric and cooperative interactions in enzymes
1Unité de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée, Centre National de la Recherche Scientifique and Aix-Marseille Université, France.
The FEBS Journal
|August 6, 2013
Summary
Seminal papers on enzyme regulation and allosteric feedback inhibition, published 50 years ago, remain highly cited today. Their foundational concepts are crucial for understanding enzyme cooperativity and systems biology.
Area of Science:
- Biochemistry
- Molecular Biology
- Systems Biology
Background:
- Key papers on allosteric feedback inhibition and enzyme cooperativity were published in the 1960s.
- These foundational works introduced groundbreaking models for enzyme regulation.
Purpose of the Study:
- To highlight the enduring significance of early research in enzyme regulation.
- To emphasize the continued relevance of these models in modern systems biology.
Main Methods:
- Historical analysis of scientific literature citations.
- Review of foundational concepts in enzyme kinetics and regulation.
Main Results:
- Papers from the 1960s on allosteric feedback inhibition and enzyme cooperativity are experiencing a resurgence in citations.
- Citations in the 21st century exceed those from the late 20th century.
Conclusions:
- The fundamental principles of enzyme regulation introduced by Monod, Changeux, Jacob, Wyman, Koshland, Némethy, and Filmer remain central to biochemistry.
- These classic studies are increasingly vital for advancements in systems biology.
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