Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Platelet proteome links metabolism to reactivity in Essential Thrombocythemia.

Molecular & cellular proteomics : MCP·2026

Same author

Residual CSB activity explains mild UV-sensitive syndrome phenotype caused by CSB mutations.

The Journal of investigative dermatology·2026

Same author

ZEB1 drives terminal erythroid maturation by controlling the GATA2-KLF1 regulatory switch.

Nucleic acids research·2026

Same author

Generation and characterization of human iPSC line SANi013-A from a Diamond-Blackfan anemia syndrome (DBAS) patient carrying a heterozygous RPS26 c.95-98 duplication variant.

Stem cell research·2026

Same author

Chromatin dynamics of the Klf4 locus in mouse pluripotent cells.

Scientific reports·2026

Same author

IFN-I-Mediated Transcriptional Reprogramming Drives Myeloid-Skewed Hematopoiesis in Sickle Cell Anemia.

American journal of hematology·2026

Search research articles

Related Experiment Video

Updated: May 9, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Locus-specific proteomics by TChP: targeted chromatin purification.

Farzin Pourfarzad¹, Ali Aghajanirefah, Ernie de Boer

¹Department of Cell Biology, Erasmus MC, Dr. Molewaterplein 50, 3015GE Rotterdam, the Netherlands; Center for Biomedical Genetics, Erasmus MC, Dr. Molewaterplein 50, 3015GE Rotterdam, the Netherlands.

|August 6, 2013

Summary

Researchers identified transcription factors controlling the repressed γ-globin gene using targeted chromatin purification (TChP). Knockdown of these factors in erythroid cells induced γ-globin expression, offering potential therapeutic targets for blood disorders.

More Related Videos

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Published on: June 1, 2018

Related Experiment Videos

Last Updated: May 9, 2026

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Published on: June 1, 2018

Area of Science:

Molecular Biology
Genetics
Hematology

Background:

The γ-globin gene is crucial for fetal hemoglobin production.
Dysregulation of globin gene expression underlies diseases like β-thalassemia and sickle cell disease.
Identifying regulatory factors is key to developing targeted therapies.

Purpose of the Study:

To develop a method for identifying transcription factors bound to specific regulatory DNA sequences in vivo.
To identify transcription factors that repress γ-globin gene expression.
To explore the therapeutic potential of targeting these factors for blood disorders.

Main Methods:

Targeted Chromatin Purification (TChP): A novel double-pull-down strategy using a tetracycline-sensitive hook.
In vivo purification of regulatory sequences and associated proteins from mammalian cells.
Validation of transcription factor binding and functional assessment via knockdown experiments.

Main Results:

TChP successfully identified transcription factors bound to repressed γ-globin gene regulatory regions.
Knockdown of several identified transcription factors in human primary erythroid cells led to increased γ-globin gene expression.
This demonstrates a direct role for these factors in repressing γ-globin.

Conclusions:

Targeted Chromatin Purification (TChP) is an effective method for identifying in vivo transcription factor binding.
Specific transcription factors repress γ-globin gene expression in erythroid cells.
These identified factors represent promising therapeutic targets for treating β-thalassemia and sickle cell disease.