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Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

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Related Experiment Video

Updated: May 9, 2026

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Wnt signaling inhibits CTL memory programming.

Zhengguo Xiao¹, Zhifeng Sun, Kendra Smyth

¹Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742 USA.

Molecular Immunology

|August 6, 2013

Summary

This summary is machine-generated.

Wnt signaling inhibits interleukin-12 (IL-12)-driven cytotoxic T lymphocyte (CTL) memory programming by suppressing T-bet and mTOR pathways. This impairment can be partially reversed with rapamycin, suggesting Wnt signaling as a target for immune therapies.

Keywords:

Cytokines Cytotoxicity GSK3 Memory Rapamycin T cells Wnt mTOR

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Published on: May 6, 2019

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Last Updated: May 9, 2026

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8⁺ T Cell Responses

Published on: May 6, 2019

Area of Science:

Immunology
Molecular Biology
Cancer Therapy

Background:

Functional cytotoxic T lymphocytes (CTLs) are crucial for vaccine development and cancer therapy.
While inflammatory cytokines drive memory CTL generation, the role of Wnt signaling in this process remains unclear.

Purpose of the Study:

To investigate the role of Wnt signaling in cytokine-driven memory CTL programming.
To elucidate the molecular mechanisms underlying Wnt signaling's impact on CTL memory.

Main Methods:

Investigated Wnt signaling's effect on IL-12-induced CTL activation and memory programming.
Analyzed the expression of transcription factors T-bet and eomes in CTLs.
Assessed the involvement of the mTOR pathway and the effect of rapamycin.

Main Results:

Wnt signaling inhibited IL-12-driven CTL activation and memory programming.
This inhibition was associated with increased eomes and decreased T-bet expression.
Wnt signaling suppressed the mTOR pathway, and rapamycin partially rescued memory programming.

Conclusions:

Crosstalk between Wnt and IL-12 signaling impairs CTL memory programming via T-bet and mTOR inhibition.
Rapamycin can partially restore Wnt-impaired CTL memory.
Wnt signaling modulation offers a potential strategy for immune therapy and managing autoimmune diseases.