Related Experiment Video

Updated: May 9, 2026

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
06:02

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Published on: February 20, 2017

Role of tumor necrosis factor alpha-induced protein 1 in paclitaxel resistance

Y Zhu1, Z Yao1, Z Wu2

  • 1Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Anhui, China.

Oncogene
|August 6, 2013
PubMed

Insights

Increased tumor necrosis factor alpha-induced protein 1 (TNFAIP1) expression causes acquired resistance to paclitaxel by interfering with tubulin polymerization. TNFAIP1 represents a potential therapeutic target for overcoming paclitaxel resistance in epithelial cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Paclitaxel is a key chemotherapy agent for epithelial cancers.
  • Acquired resistance significantly limits paclitaxel's long-term efficacy.
  • Understanding resistance mechanisms is crucial for developing improved cancer therapies.

Purpose of the Study:

  • To identify novel genes associated with acquired paclitaxel resistance.
  • To elucidate the functional role of TNFAIP1 in paclitaxel resistance.
  • To explore TNFAIP1 as a potential therapeutic target.

Main Methods:

  • Investigated TNFAIP1 expression in paclitaxel-resistant cancer models.
  • Examined the interaction between TNFAIP1 and β-tubulin.
  • Assessed the impact of TNFAIP1 modulation on paclitaxel sensitivity in vitro and in vivo.
  • Analyzed TNFAIP1 regulation by Sp1.

Main Results:

  • Increased TNFAIP1 expression confers acquired resistance to paclitaxel.
  • TNFAIP1 competes with paclitaxel for β-tubulin binding, inhibiting polymerization and cell death.
  • Sp1 transcriptionally regulates TNFAIP1 expression.
  • In vivo studies showed TNFAIP1 overexpression reduces tumor response, while knockdown enhances it.

Conclusions:

  • TNFAIP1 is a novel gene conferring paclitaxel resistance.
  • TNFAIP1's mechanism involves direct interference with paclitaxel's action on tubulin.
  • Targeting TNFAIP1 may restore sensitivity to paclitaxel in resistant cancers.

More Related Videos

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel06:44

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Published on: September 8, 2017

Related Concept Videos

Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...