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Updated: May 9, 2026
07:20
Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Planifilum composti sp. nov., a thermophile isolated from compost
Song-Ih Han1, Jae-Chan Lee2, Hyo-Jin Lee1
1Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, Doan-dong 800, Seo-gu, Daejeon 302-729, Republic of Korea.
Summary
Two new thermophilic bacteria were discovered in Korean compost. These bacteria represent a novel species, Planifilum composti, expanding the known diversity of thermophilic microorganisms.
Area of Science:
- Microbiology
- Bacteriology
- Genomics
Background:
- Thermophilic bacteria are crucial for organic matter decomposition in compost environments.
- Understanding microbial diversity in compost aids in optimizing composting processes and identifying novel microorganisms with industrial potential.
Purpose of the Study:
- To isolate and characterize novel thermophilic bacteria from compost.
- To determine the taxonomic and phylogenetic placement of the isolated strains.
- To propose a new species within the genus Planifilum.
Main Methods:
- Isolation and cultivation of thermophilic bacteria from compost samples.
- Physiological and biochemical characterization of the isolates.
- Analysis of cell wall components, menaquinone, and fatty acid profiles.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- DNA-DNA hybridization for taxonomic delineation.
Main Results:
- Two aerobic thermophilic strains, P8(T) and P12, were isolated, growing between 50-75 °C.
- The isolates produced single spores and lacked aerial mycelia.
- Phylogenetic analysis placed the strains within the genus Planifilum, with 96.1-97.2% 16S rRNA gene sequence similarity.
- Low DNA-DNA relatedness (28.9-38.2%) indicated they represent a new species.
- The predominant menaquinone was MK-7, with major fatty acids iso-C17:0, iso-C15:0, and iso-C16:0.
Conclusions:
- Strains P8(T) and P12 represent a novel species of the genus Planifilum.
- The proposed name for this new species is Planifilum composti sp. nov.
- This discovery contributes to the understanding of thermophilic bacterial diversity in composting ecosystems.
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